phosphorylated tyrosine sites  (R&D Systems)

Journal: International Journal of Molecular Sciences

Article Title: Tobacco Smoke Condensate Induces Morphologic Changes in Human Papillomavirus-Positive Cervical Epithelial Cells Consistent with Epithelial to Mesenchymal Transition (EMT) with Activation of Receptor Tyrosine Kinases and Regulation of TGFB

doi: 10.3390/ijms25094902

Figure Lengend Snippet: CSC effects on the expression of RTK activation upregulating downstream effector protein, MAPK, in human cervical epithelial cells. ( A ) Expression of growth factor RTKs in HPV-16 Ect/E6E7 cells shown by representative RTK array (top) and as quantitated dot blot intensity (0 and 10 µg/mL) values (mean + SE) at the bottom. ( B ) Western blotting for phosphorylated MAPK44/42. Data are represented as mean ± SE; * p < 0.05 compared with the control group. Significance was expressed as mean ± SE of at least three replicates.

Article Snippet: The array was conducted following the manufacturer’s instructions to detect phosphorylated tyrosine sites on RTKs (#ARY001B, R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Activation Assay, Dot Blot, Western Blot, Control

Journal: JID innovations : skin science from molecules to population health

Article Title: Identification of Collagen-Suppressive Agents in Keloidal Fibroblasts Using a High-Content, Phenotype-Based Drug Screen.

doi: 10.1016/j.xjidi.2023.100248

Figure Lengend Snippet: Figure 7. CGP60474 and MAPK signaling. (a) Representative phosphokinome array for KF-1762 (Proteome Profiler—Human Phospho- Kinase Array—(R&D Systems, ARY003C) showing most upregulated phosphoproteins (p-ErkT202/Y204,T185/

Article Snippet: Phosphorylated kinase array and phosphorylated RTK array Proteome Profiler Human Phospho-Kinase Array Kit (ARY003C) and Proteome Profiler Human Phospho-RTK Array Kit (ARY001B) were purchased from R&D Systems (Minneapolis, MN).

Techniques:

Journal: Molecular Cancer

Article Title: Ceritinib is a novel triple negative breast cancer therapeutic agent

doi: 10.1186/s12943-022-01601-0

Figure Lengend Snippet: ACK1 is a major Ceritinib target in MDA-MB 453. ( A ) The levels of phosphorylated tyrosine kinases in a panel of 73 RTKs were analyzed by a human RTK Phosphorylation Antibody Array C1 Kit (RayBiotech) after MDA-MB 453 cells were treated with 10 μM ceritinib, DMSO was used as control. The positive dot#1,#2,#3 are all controlled amount of biotinylated antibody, which is consistent from array to array. As such, the intensity of these Positive Control signals serves much like housekeeping proteins. ( B ) The densitometric values of phospho-RTKs were determined by an image lab software (Bio-Rad). The relative intensities of the duplicated spots were normalized to positive control 1 spots. Values represent the mean of duplicate spots for each protein after normalization. ( C ) AR and ACK1 were determined by WB assay in human breast cancer cell lines and ( D ) in human breast cancer tissues

Article Snippet: To determine which receptor tyrosine kinases (RTKs) are targeted by ceritinib, the Human RTK Phosphorylation Antibody Array C1 Kit (RayBiotech, Cat. No. AAH-PRTK-1-2) was used.

Techniques: Ab Array, Positive Control, Software